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Cover page of the Journal of Health Sciences
ORIGINAL ARTICLE
Year : 2019  |  Volume : 12  |  Issue : 2  |  Page : 112-116

Cytotoxic activity of endophytes isolated from Allamanda blanchetii A. DC


1 Department of Ethanomedicine, National Institute of Traditional Medicine (Indian Council of Medical Research), KLE University; Department of Cell Culture and Microbiology, MMDS, Belagavi, Karnataka, India
2 Department of Ethanomedicine, National Institute of Traditional Medicine (Indian Council of Medical Research), KLE University, Belagavi, Karnataka, India
3 Department of Microbiology, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India

Correspondence Address:
Dr. Harsha V Hegde
National Institute of Traditional Medicine (Indian Council of Medical Research), Nehru Nagar, Opposite to KLE University, Belagavi - 590 010, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/kleuhsj.kleuhsj_7_19

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AIM: To evaluate the cytotoxic potential of endophytic fungi isolated from A. blanchetii. MATERIALS AND METHODS: Endophytic fungi were isolated from A. blanchetii using surface sterilization methods, grown in different types of media to ascertain their suitability for growth. Mass culture was prepared using suitable media and extracts using several solvents were prepared and dried. These extracts were later evaluated using MTT assay for their cytotoxic potential. RESULTS: Hydroalcoholic extract of Phomopsis species exhibited better cytotoxicity (80 μg/ml) when compared ethyl acetate extract of the same 160 μg/ml. Similarly, ethyl acetate extract of Phomopsis species, ethyl acetate extract of Alternaria aff. raphani J. W. Groves and Skolko also demonstrated cytotoxicity of 160 μg/ml in MTT assay against MCF 7 cell line. CONCLUSION: Both the species of Phomopsis and Alternaria have shown significant cytotoxicity in MTT assay.


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